Equipment development of real-time monitoring device for intestinal host- microbial interaction
- Publication Date: 2020-04-03
| Execution Methods | Transepithelial electrical resistance (TER) is a widely used quantitative technique to measure the changes of tight junction-mediated barrier function in intestinal epithelial monolayers. TER values could reflect intestinal physical barriers changes when co-culture the cells with specific nutrients, microbial products, drugs, or chemicals. However, several limitations exist in the current technique for TER measurement. To date, it has not been possible to measure the real-time TER changes, especially when the cells cultured in specific atmospheric conditions. It is also challenging to collect big data when using classic TER recording methods. In the current project, we aim to development of a wireless sensor system for real-time monitoring of TER measurement. |
| Performance Evaluation | The single-side printing (cis-electrode) flexible printed circuit (FPC) electrodes, which are similar to the design of traditional electrode, were used in our pilot test. The long-term recording of TER values by FPC are stable in the cell media- containing transwells. Moreover, our output TER signal was connected to an Arduino Wi-Fi system and the data was imported to cloud-based googleâ sheetsâ. The data could be download from remote computer successfully. The innovative device will provide several advantages for TER recording. The abstract of pilot results has been submitted to the 2021 Joint Annual Conference of Biomedical Science. |
| Conclusion & Suggestion | Our innovative design will bring unprecedented insights into the field of electrophysiological study in gastrointestinal epithelial permeability and barrier function assessment. We will file the patent application for a claimed invention. |
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