| Application Dept. |
Dept. of Bio-Industrial Mechatronics Engineering |
| Principal Investigator |
Prof. Ching-Chou Wu ( Personal Webpage ) |
| Project Title |
An ultrasensitive and label-free impedimetric immunosensor for the rapid detection of foodborne bacteria |
| Co-Principal Investigator |
Prof. Chiou-Ying Yang, G. I. of Molecular Biology |
| Co-Investigator |
|
| Abstract |
Salmonella, being one of bacteria causing foodborne illness, has the highest mortality in food poisoning. Therefore, developing a sensitive and rapid method for detecting bacteria is an important issue in the sanitary field. This study proposes a label-free impedimetric biosensing technique with immune magnetic nanoparticles (MNPs) for enrichment, separation and detection of Salmonella. The MNPs modified 7F1A antibody (Ab1) reacts with Salmonella for separation and enrichment, which can increase the concentration of bacteria and prevent the effect of interference. Salmonella/Ab1@MNPs complexes are transported to the detector region by alternating current electrokinetic flow (ACEKF) and are concentrated to the surface of detector by dielectrophoresis (DEP), which can increase immunoreaction rate, shorten response time, lower the detection limit of bacteria and improve sensitivity. The label-free impedimetric immunosensing platform of this study for the detection of foodborne bacteria can be used not only in food safety field but also in other immunoassay applications. |
