利用列當親緣地理與植體代謝物間關係建立代謝物可攜式快速分析平台

  • 刊登日期: 2022-02-25
申請系所(單位) 生命科學系
計畫主持人 黃盟元 副教授 ( 個人網頁 )
計畫名稱(中文) 利用列當親緣地理與植體代謝物間關係建立代謝物可攜式快速分析平台
計畫名稱(英文) Utilizing the relationship between phylogeny and metabolites to establish a portable and rapid analysis platform for metabolites on Orobanche caerulescens
共同主持人 1. 林柏亨 教授 / 化學系 2. 盧臆中 助理教授 / 化學系 3. 曾妤馨 助理教授 / 生科系
協同主持人
中文摘要 列當(Orobanche caerulescens Stephan ex Willd),是列當科(Orobanchaceae)列當屬植物,需要寄生在茵陳蒿(Artemisia capillaris)之根部才能生長。台灣列當其族群明顯地呈現斷續分布的情況,這類的植物分布模式,一直是生物地理所關心的議題,但對於台灣所產列當的分布模式及族群結構,都尚待釐清。近代藥理學研究指出列當其主要成分苯乙醇苷類(phenylethanoid glycosides;PhGs)具保肝、抗氧化、抗衰老、神經保護、免疫調節等功效,並以含量最高之松果菊苷(Echinacoside;ECH)作為品管標準。列當在台灣非藥食兩用藥材,中醫師應沒有在使用列當,但列當在台灣早已被原住民利用,亦有民間團體栽培販售,故建立其植體成分分析資料作為未來合法使用的參考依據相當的重要。本計畫將採集全台不同地點的列當,比較其親緣關係及環境因子對其代謝產物的影響,作為後續培養與藥用之基礎。我們擬結合本校化學系開發的新穎質譜快篩平台分析代謝產物,此快篩平台應用最新的基質輔助游離法(matrix-assisted ionization),使樣本無需複雜前處理即可不受生物體內複雜環境的干擾直接探測目標代謝物。除了大幅簡化傳統質譜法的分析流程外,本平台安裝於可攜式質譜儀後分析場域將不再局限於實驗室內,能在不同採集點迅速得到初步分析結果。本計畫整合新穎分析平台及生物學,研究具潛力之藥用植物,不論在基礎生物研究或產業應用都具有相當的國際競爭力。
英文摘要 Orobanche caerulescens is a plant belonging to the family Orobanchaceae, and needs to parasitize upon the roots of Artemisia capillaris. Obviously, the O. caerulescens are discontinuously distributed. This distribution pattern has always been a topic of concern in Biogeography, but the distribution pattern and population structure in Taiwan have not to be clarified. The pharmacological studies have pointed out that the main components of O. caerulescens are phenylethanoid glycosides (PhGs), which enhance liver protection, anti-oxidation, anti-aging, neuro-protection and immune regulation. The Echinacoside acts as a reference standard. In Taiwan, O. caerulescens is considered neither food nor medicine. The conventional Chinese physician should not be using it; however, it has long been used by the indigenous in Taiwan. It is also cultivated and merchandised by private institutions. It is very important to establish the statistical database of its implant components as a referent legal grounding. This project will collect O. caerulescens from different locations in Taiwan, and compare the relationship of environmental factors and metabolites, in order to set up a baseline for further cultivation and pharmaceutical use. We would like to study its metabolic products using a portable mass spectrometer developed by our Chemical department. The matrix-assisted ionization method is applied to directly study the subject’s metabolic products without affecting from the subject’s biological interventions. Not only simplifies the analysis procedure, the portable mass spectrometer also works outside the laboratory. It allows us to collect samples and get the initial test result on site. This project examines a potential medicinal plant using a novel analytical tool and Biology. It is highly competitive globally in both fundamental Biology study and industrial application.